Urine Culture
1. Objective To detect and identify pathogenic bacteria (or fungi) in urine and determine their antibiotic susceptibility , especially in suspected urinary tract infections (UTIs) . 2. Principle Urine is inoculated onto differential and selective media to isolate and identify uro-pathogens such as E. coli , Klebsiella , Proteus , and Enterococcus . A colony count is performed to assess the significance of bacterial growth, and susceptibility testing is carried out using methods like Kirby-Bauer disc diffusion . 3. Materials Sterile midstream urine sample Inoculating loop (1 µL or 10 µL calibrated) Culture media: Cystine Lactose Electrolyte Deficient (CLED) agar MacConkey agar Blood agar Incubator (35–37°C) Antibiotic discs (for sensitivity testing) Mueller-Hinton agar (for disc diffusion) PPE and sterile containers 4. Procedure Mix the urine sample gently. Use a...